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Angiogenesis is essential for the growth of solid tumors. Recent experimental data indicate that several biochemical markers are linked to angiogenesis in malignant melanoma. The purpose of this study was to examine the relationship between the histopathological features of malignant melanoma and the expression of these markers.

Methods
=======

Paraffin embedded tissue from 10 cases of malignant melanoma were retrieved from the archives of the Department of Pathology of the Chaim Sheba Medical Centre, Israel. The expression of the following markers were evaluated: MMP-9, VEGF, MHC class I, CD8, and CD31. Histopathological features were examined and scored as per the S-100 and melanoma differentiation markers.

Results
=======

1\. The mean age of the patients was 61.5 years. The scalp was the commonest location for the tumors. Histologically, all tumors contained nests of malignant melanocytes, which were admixed with varying numbers of mature lymphocytes and fibroblasts. Cytoplasmic S-100 and HMB-45 staining was observed in the malignant melanocytes in all cases.

2\. A predominant invasion pattern was present in 6/10 cases. The tumor thickness was measured and consisted of a wide range (2.0–5.9 mm). The Breslow’s thickness was determined in 4 cases. The Clark level was measured in 3 cases and was below 2 mm in all cases. The invasion depths of the tumor were classified as follows: 5/10 cases were classified as M1 invasion and the remaining 5/10 cases were M0.

3\. The mean score for the S-100 staining was 4.9 (range 3 to 5) with high staining intensity. The mean score for the MHC class I staining was 6.3 (range 4 to 7) with high staining intensity. The mean score for the VEGF staining was 2.5 (range 2 to 4) with high intensity staining. The mean score for the CD8 staining was 6.3 (range 3 to 7) with high staining intensity. The mean score for the CD31 staining was 9.6 (range 6 to 10) with high staining intensity.

4\. The expression of MMP-9 and

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